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KMID : 1234920110110010035
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Journal of Korean Oriental Association for Study of Obesity
2011 Volume.11 No. 1 p.35 ~ p.46
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Antimicrobial Effects of Fermented Coptidis rhizoma and Lonicerae Flos against pathogen
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Lee Sin-Ji
Lee Myeong-Jong
Jung Ji-Eun
Kim Ho-Jun
Shambhunath Bose
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Abstract
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Objectives: This study was designed to examine antimicrobial effects of Fermented Coptidis rhizoma and Lonicerae Flos against pathogens.
Methods: Lactobacilli MRS broth was added to 200mL glass bottle containing 20% herb powder(w/v) followed by 30 minute sonication and then shaking at 70 rpm in water bath for 3 hours in order to extract fermented herb. Fermented herb extract was autoclaved at for 15minutes. CFU/mL subcultured bacteria was inoculated and cultured for 24 hours and centrifuged at 3000 rpm for 5 minutes. After transferring to 15 mL conical tube, the viable cells were counted.
Results and Conclusion : Fermented Coptidis rhizoma and Lonicerae Flos both showed antimicrobial effect on pathogens especially when Fermented Coptidis rhizoma was experimented against Staphylococcus aureus.
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KEYWORD
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Fermented Herbal extracts, Coptidis rhizoma, Lonicerae Flos, Pathogens, Staphylococcus aureus, Salmonella spp.
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